Macrophages have been known to produce morokines and to involve to the cytotoxic activity against microorganisms or tumor cells.
Using murine peritoneal macrophages the effect of interferon-γ(γIEN), lipopolysaccharide (LPS), NG-monomethyl-L-arginine (NMLA) or dexamethasone in combination was assessed on the activity of interleukin-1 and tumor necrosis factor, the
production of
NO, and the cytotoxicity against murine lymphocytic leukemic cell L1210.
@ES The results were as follows;
@EN 1. The activity of interleukin-1 was increased y the treatment of combined γIFN and LPS, compared with the control. This increased activity was little decreased by adding NMLA, while being considerably decreased by adding dexamethasone.
2. The activity of tumor necrosis factor was increased in theγIFN and LPS-treated cells cells in compared with control cells. That was not affected by adding NMLA in theγIFN and LPS-treated cells, while being considerably decreased by adding
dexamethasone.
3. The production of NO was significantly increased in the cells treated with combination ofγIFN and LPS, compared with the control. By the addition of NMLA or dexamethasone in theγIFN and LPS-tieated cells, the production of NO was
considerably
suppressed.
4. The cytotoxic activity of macrophages was significantly increased in the γIFN and LPS-treated cells, compared with the control. This increased cytotoxic activity was suppressed by the addition of NMLA or dexamethasone.
These effects of NMLA indicate that the cytotoxicity of the murine macrophasges against murine lymphocytic leukemic cell may be closely related with the production of NO.
|